Fig 1: SNHG3 influences growth of solid tumor and methionine dependence of PCa in nude mice. A Tumor image and tumor weight dissected from nude mice 35 days later (N = 3, *p < 0.05); B tumor growth curve (N = 3, *p < 0.05); C, D SNHG3, miR-152-3p, and SLC7A11 levels in tumor tissue were assessed via qRT-PCR and western blot. The experiment was repeated 3 times, *p < 0.05; E percentage of Ki-67 positive cells in xenogeneic tumor tissue assessed by IHC (scale bar: 50 µm). The experiment was repeated 3 times, *p < 0.05; F tumor weight ratio of nude mice at terminal stage with a normal diet or a diet lacking MET (MET-HCY+ diet) (N = 3, *p < 0.05)
Fig 2: Erianin suppressed tumor growth by inducing ferroptosis in vivo. (A) Representative image of mice and xenograft tumors at the endpoint of experiment. (B) The average tumor volume from different groups. Values are shown as mean ± SD, **p < 0.01. (C) The average mice weight from different groups. Values are shown as mean ± SD. (D) The expression of Ki67, GPX4, SLC40A1 and xCT were detected by IHC.
Fig 3: PPI and the co-regulatory network of SLC7A11. (A) SLC7A11-interaction proteins in COAD tissue. The network consists of 11 nodes and 23 edges, the red nodes are involved in the ferroptosis signaling pathway. (B) TF-miRNA co-regulatory network. The network consists of 270 nodes and 397 edges. The nodes in red color are the co-expression genes, the blue nodes represent miRNA, the green nodes represent TFs, and the yellow nodes represent protein.
Fig 4: Genes analysis of closely associated with SLC7A11 in COAD. (A) The top 50 genes correlated with SLC7A11 in COAD are showed in the heatmap; ***p < 0.01. (B) GO–KEGG enrichment analysis of co-expression genes with SLC7A11 in COAD.
Fig 5: Influence of SAL on iron metabolism in diabetic mice. (A), The serum iron levels of mice in the different groups; (B), The transferrin levels of mice in the different groups; (C) Western blot analysis of the protein expression of SLC7A11, LC3II, and GAPDH in myocardial tissue; (D) Grey value analysis of the western blot results. (ns, no statistical significance; *p < 0.05 as MD group vs WT group; **p < 0.05 as MD group vs SAL group). ***means that SAL group compared with WT group is difference significant.
Supplier Page from Abcam for Anti-xCT antibody [EPR8290(2)]